Process-scale production typically involves more than one column. The packing quality of each column affects the efficiency of the whole workflow and is an important validation criterion. Here, we investigate the effects of packing methods, packing solutions, compression factor, resin slurry concentration, and bed height on the efficiency of packed columns.

There is a growing need to close and automate manufacturing of chimeric antigen receptor (CAR)-T cell therapies. CAR-T workflows depend on ex vivo gene transfer by lentiviral transduction for therapeutic efficacy. To streamline this step while keeping flexibility during process development, we introduced stand-alone SpinOculation C-Pro protocol software.

Experts at Cytiva provide insights into implementing a robust business continuity management program that can help secure supply, reduce risks, and maintain quality in manufacturing operations, through a crisis situation.

Is your CDMO an asset or a hindrance? This article explores 10 red flags that signal your parter isn't carrying its weight on your journey to commercialization.

Using a design of experiments approach, this study describes the effect of buffer pH and NaCl concentration on proteins purified by size exclusion chromatography.

Biomanufacturing is trending towards higher numbers of monoclonal antibody (mAb) projects and smaller batch sizes, with production of most mAbs below 100 kg/yr. These trends are fueling demands to screen more clones faster and improve the efficiency of process development (PD). Read how the Fibro PrismA unit provides a time advantage with comparable recovery and product quality attributes to resin-based column.

Refocusing R&D strategies towards diagnostics and considering industry partnerships should be key considerations when developing enterprise risk plans during a crisis like COVID-19. 

qPCR remains the gold standard for validation of microarray and next generation sequencing data and the method of choice for both clinical and basic research labs for a wide range of applications. However, there remains general concern about the production of data that truly reflects the tested experimental conditions. We have developed a comprehensive guide to performing the ultimate qPCR experiment. The following is a snapshot of the critical steps needed to achieve excellent results.

RT-qPCR is a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics, as well as food safety. There are multiple benefits of this technology. In this application note, we demonstrate a sensitive codetection of viral RNA and DNA targets in a multiplex setting using a novel one-step multiplex RT-qPCR supermix.

Protein thermal shift assays enable quick and easy buffer optimization for increased protein stability. Learn more about the CFX Real-Time PCR Detection Systems which uses a simple protocol to measure protein thermal stability using SYPRO Orange Fluorescent Dye.