Product/Service

Sulforhodamine Caspase Detection Kit

Source: Cell Technology, Inc.
Red fluorescent emitting peptide inhibitors for detection of active caspases in living cells.
Unique:
Other caspase detection assays on the market work through substrate cleavage mechanisms or immunohistochemical principles. APO LOGIX reagents work by active caspase inhibition. Since apoptosis is arrested upon the binding of the reagent to the active caspase heterodimer, the apoptotic cell population does not diminish over time.

Easy to Use:
Add reagent directly to cells. No special buffer or media needed. No preparation of cell lysates required. Simple wash procedure.

Quick:
Incubate for one hour, wash twice, and measure. No time course studies necessary.

Adaptable:
Works in diverse cell lines: human, rodent, Drosophila. Can be performed in conjunction with Annexin staining, TUNEL, antibody staining, or with other APO LOGIX reagents on the same population of cells. Permits high through-put screening. Protocol can be adapted for ex vivo as well as in situ experiments.

Non-Cytotoxic:
Arrests further apoptotic activity via caspase inhibition.

Cell Permeable:
Permits direct visualization of cytosolic apoptotic events.

Compatible with Multiple Fluorometric Modalities:
Fluorescence microscopy. 96-well fluorescence plate reader.

Reliable:
Yields both quantitative and qualitative results. Gives strong signal with little background noise.

Optimizable:
Cell Technology offers free technical support to help theresearcher optimize the application of the APO LOGIX reagents to particular experimental contexts.

Comprehensive:
APO LOGIX reagents mark activity across the range of caspase proteins. Our poly-caspase assay is useful for visualizing general caspase activity.

APO LOGIX
Sulforhodamine caspase detection kits label active caspases in living cells that are undergoing apoptosis. Cell Technology's probes utilize Sulforhodamine (SR) labeled peptide fluoromethyl ketone (FMK) caspase inhibitors (SR-peptide- FMK). These SR-peptide-FMK are both cell permeable and non-cytotoxic during the course of the assay, thus allowing the detection of active caspases in living cell systems.

Assay Principle:
When added to a population of cells undergoing apoptosis, the SR-peptide-FMK probe enters each cell and covalently binds to the reactive cysteine residue on the large subunit of the caspase heterodimer, thereby the probe is sequestered and accumulates inside the cell. At the same time it inhibits further caspase activity. The remaining unbound reagent is easily washed away. Cells with the bound SR-peptide-FMK can be detected by flow cytometry, fluorescence microscope or a 96 well fluorescence plate reader. The SR-peptide-FMK reagent excites at 550 nm and has a maximum emission range of 590-600 nm.

Cell Technology, Inc., 2010 E. Hennepin Ave - Suite 203 , Minneapolis, MN 55413. Tel: 612-331-4704; Fax: 612-378-2319 .