Product/Service

Carboxyfluorescein Caspase Detection Kits

Source: Cell Technology, Inc.
Apo Logix Carboxyfluoroscein Caspase Detection Kits label active caspases in living cells undergoing apoptosis
Apoptosis detection and in situ labeling of active caspase in live cells.

Apo Logix Carboxyfluoroscein Caspase Detection Kits label active caspases in living cells undergoing apoptosis. Cell Technology's probes utilize carboxyfluorescein(FAM)-labeled peptide fluoromethyl ketone (FMK) caspase inhibitors (FAM-peptide-FMK). These FAM-peptide-FMK compounds are both cell permeable and non-cytotoxic during the course of the assay and thus allow the detection of active caspases in living cell systems.

Other caspase detection assays on the market work through substrate cleavage mechanisms or immunohisto-chemical principles. APO LOGIX reagents work by active caspase inhibition. Since apoptosis is arrested upon the binding of the reagent to the active caspase heterodimer, the apoptotic cell population does not diminish over time.

Unique
Other caspase detection assays on the market work through substrate cleavage mechanisms or immunohisto-chemical principles. APO LOGIX reagents work by active caspase inhibition. Since apoptosis is arrested upon the binding of the reagent to the active caspase heterodimer, the apoptotic cell population does not diminish over time.

Easy to Use
Add reagent directly to cells. No special buffer or media needed. No preparation of cell lysates required. Simple wash procedure.

Quick
Incubate for one hour, wash twice, and measure. No time course studies necessary.

Adaptable
Works in diverse cell lines: human, rodent, Drosophila. Can be performed in conjunction with Annexin staining, TUNEL, antibody staining, or with other APO LOGIX reagents on the same population of cells. Permits high through-put screening. Protocol can be adapted for ex vivo as well as in situ experiments.

Non-Cytotoxic
Arrests further apoptotic activity via caspase inhibition.

Cell Permeable
Permits direct visualization of cytosolic apoptotic events.

Compatible with Multiple Fluorometric Modalities
Fluorescence microscopy. 96-well fluorescence plate reader. Flow cytometry.

Reliable
Yields both quantitative and qualitative results. Gives strong signal with little background noise.

Optimizable
Cell Technology offers free technical support to help the researcher optimize the application of the Apo Logix reagents to particular experimental contexts.

Comprehensive
APO LOGIX reagents mark activity across the range of caspase proteins. Our poly-caspase assay is useful for visualizing general caspase activity. We also have caspase assays to target caspases 1, 2, 3, 6, 8, 9, or 10.

Cell Technology, Inc., 2010 E. Hennepin Ave - Suite 203 , Minneapolis, MN 55413. Tel: 612-331-4704; Fax: 612-378-2319 .